You are invited to the New England Society for Microscopy's 32nd Annual Spring Workshops at the Marine Biological Laboratory on Thursday, April 30th! Below, you can find our workshop lineup and thee schedule for the day. Registration for the Symposium on Friday, May 1st, as well as housing and parking information, can be found here
Schedule
1:00 PM – Welcoming Remarks - Louie Kerr, Treasurer
1:10 PM – Workshops Part I
2:30 PM – Afternoon Coffee Break
3:00 PM – Workshops Part II
5:00 PM – Closing
Workshop Descriptions
“Expansion Microscopy” , Fei Chen & Paul Tillburg, Massachusetts Institute of Technology
The Expansion Microscopy (ExM) workshop will give an introduction to the ExM process, along with a hands-on tutorial for sample preparation, handling, and expansion of cells and tissues. Find out more about ExM at http://expansionmicroscopy.org/.
“Basic Light Microscope Maintenance” , Lauren Alvarenga, Harvard Medical School
The Basic Light Microscope Maintenance workshop is designed for those of you who are interested in learning theoretical and practical knowledge about maintaining a light microscope for quantitative microscopy. This workshop will take you through the maintenance of both transmitted light and fluorescence microscopy and will apply to a wide variety of imaging systems so that you can take what you have learned and apply it to real-world experiences. Topics will include microscope evaluation, corrective actions for common optical problems, and cleaning techniques for objective lenses and filter sets. Following a lecture presentation, participants will gain hands-on experience with a microscope and various parts along with test sample preparation to help evaluate the microscope light path.
“Imaging and Force Measurements with Optical Tweezers”, Dr. Jenny Tam, MGH
Would you like to non-invasively manipulate and position microscopic objects using only light? Come and learn about optical tweezers and see how they can transform how you think about your experiments and research. Not only can optical tweezers control objects in solution, they can also apply mechanical force. The applied force can be quantified, and combining optical tweezers with imaging allow visualization of the biological interaction between two trapped objects. You’ll learn how optical tweezers are set up, calibrated, and how they can be combined with techniques like confocal microscopy and FRET. We will have "hands-on” work with the instrument to manipulate objects and calibrate the optical tweezers for force measurements.